You can subtract multiple cells from one cell in Excel by using Minus sign, SUM function and Paste Special feature. Here is a tutorial on this.

Launch Excel or open an existing table with numbers to add. Type the calculation into the cell you want to place the result, for eg =15+25+4+10+5, Press the Enter key to see the result. 2] How to ...

Like we already mentioned, there’s no actual command in Excel for subtracting values, you have to use an operator in a formula. That said, we can create individual formulas in cells.