This Perspective discusses the development of the linear amplified RNA amplification technique over the last 25 years, and future applications of this important and versatile methodology.

Abstract Linear amplification for deep sequencing (LADS) is an amplification method that produces representative libraries for Illumina next-generation sequencing within 2 d.

WT-Ovation™ utilizes an amplification approach that is based on NuGEN's Ribo-SPIA™ technology, achieving an average of 1,500-fold linear amplification of mRNA.